製品の概要

  • 製品名
    Anti-Cy3 / Cy5 antibody [CY96]
  • 製品の詳細
    Mouse monoclonal [CY96] to Cy3 / Cy5
  • アプリケーション
    適用あり: Dot blot, ELISA, IP, ICC, Other, ICC/IFmore details
  • 免疫原

    A mixture of proteins labelled with Cy3 or Cy5.

  • 特記事項


    Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • 特記事項(精製)
    Purified Mouse Immunoglobulin
  • 一次抗体 備考
    Monoclonal antibodies to Cy3 and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.
  • ポリ/モノ
    モノクローナル
  • クローン名
    CY96
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use a concentration of 1 - 2 µg/ml.
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Other Use at an assay dependent concentration.

Protein microarrays

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 関連性
    Cy3 and Cy5 are reactive watersoluble fluorescent dyes of the cyanine dye family used for labelling proteins, peptides, DNA, RNA, and other biomolecules. They are usually synthesized with reactive groups on either one or both of the nitrogen side chains so that they can be chemically cross-linked to nucleic acids or protein molecules. Thay are small, pH insensitive (between pH 3 and pH 10), soluble in aqueous solution and have tolerance to DMSO. They are more photostable than fluorescein, have high molar extinction coefficients and favorable quantum yields. They are used in many different biological assays, including DNA microarrays, protein microarrays, two-dimensional protein analysis, fluorescence resonance energy transfer, and immunocytochemistry. Cy3 is excited maximally at 550 nm and emits maximally at 570 nm, in the green part of the spectrum. Cy5 is excited maximally at 649 nm and emits maximally at 670 nm, in the red part of the spectrum.

参考文献

This product has been referenced in:
  • Osterwald S  et al. PML induces compaction, TRF2 depletion and DNA damage signaling at telomeres and promotes their alternative lengthening. J Cell Sci 128:1887-900 (2015). Read more (PubMed: 25908860) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Specification
U2OS
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2 % Triton X 100 in PBS for 12 min
Blocking step
Goat serum in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Feb 25 2013

Thank you for contacting us.

Unfortunately we do not have any images of product ab52061 and ab52060 with Alexa 647. Alexa 647 is based on the structure of Cy5 and therefore is structurally very similar. As our data sheet states this antibody...

Read More

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

Read More

Thank you for confirming these details. I am sorry to hear that these antibodies did not work in ICC/IF.
As requested, I have issued a free of charge replacement of ab3851, ab52060, and ab112511. These will be shipped out tonight for delivery tomor...

Read More

Thank you for contacting us. I am sorry we are not expert in this field. However we have few recommendations that will help you to search the right antibodies. I have attached a FRET presentation which I found while searching on Google. Could you p...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録