製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CXCR3
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human CXCR3.

    (Peptide available as ab74828.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human tissue lysates: Colon, Small Intestine, Lymph Node and Heart IF/ICC: HeK293 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC).
    Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). May play a role in angiogenesis.
    Isoform 3 mediates the activity of CXCL11.
  • 組織特異性
    Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-X-C chemokine receptor type 3 antibody
    • CD 183 antibody
    • CD182 antibody
    • CD183 antibody
    • Chemokine (C X C motif) receptor 3 antibody
    • Chemokine (C X C) receptor 3 antibody
    • Chemokine CXC Motif Receptor 3 antibody
    • CKR L2 antibody
    • CKR-L2 antibody
    • CKRL2 antibody
    • CMKAR3 antibody
    • CXC-R3 antibody
    • CXCR-3 antibody
    • CXCR3 antibody
    • CXCR3_HUMAN antibody
    • G Protein Coupled Receptor 9 antibody
    • G protein-coupled receptor 9 antibody
    • GPR9 antibody
    • Interferon-inducible protein 10 receptor antibody
    • IP-10 receptor antibody
    • IP10 antibody
    • IP10 R antibody
    • IP10 receptor antibody
    • IP10-R antibody
    • IP10R antibody
    • Mig R antibody
    • Mig receptor antibody
    • Mig-R antibody
    • MIGR antibody
    see all

画像

  • ICC/IF image of ab71864 stained HEK293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CXCR3 antibody (ab71864) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

    Our observed 70kDa band is supported by the following publications: http://www.jbc.org/content/279/29/30219.long and http://www.jimmunol.org/cgi/content/full/173/10/6234

参考文献

This product has been referenced in:
  • O'Beirne SL  et al. CXCL9 Regulates TGF-ß1-Induced Epithelial to Mesenchymal Transition in Human Alveolar Epithelial Cells. J Immunol 195:2788-96 (2015). Read more (PubMed: 26268659) »

See 1 Publication for this product

レビューと Q&A

Abreviews
Application
Flow Cytometry
Sample
Cow Cell (lymphocytes)
Permeabilization
No
Gating Strategy
lymphocytes
Specification
lymphocytes
Preparation
Cell harvesting/tissue preparation method: Ficoll gradient
Sample buffer: optimized PBS (BD Cellwash)
Fixation
none
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投稿 Sep 18 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cow Tissue sections (spleen, lymphe nodes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citratbuffer pH6.0 / Tris-EDTA pH 9.0
Permeabilization
No
Specification
spleen, lymphe nodes
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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投稿 Aug 14 2015

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Mouse Cell lysate - whole cell (Endothelcells)
Specification
Endothelcells
Treatment
Ethanol und Dexametason 100nM 24h
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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