製品の概要

  • 製品名Anti-CXCR2 antibody
    CXCR2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CXCR2
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    A synthetic peptide mapping at the middle region of human CXCR2, different from the mouse sequence by six amino acids.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 翻訳後修飾Phosphorylated upon ligand binding; which is required for desensitization.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-X-C chemokine receptor type 2 antibody
    • CD 182 antibody
    • CD182 antibody
    • CD182 antigen antibody
    • CDw128b antibody
    • Chemokine (CXC) receptor 2 antibody
    • CMKAR2 antibody
    • CXC-R2 antibody
    • CXCR 2 antibody
    • CXCR-2 antibody
    • CXCR2 antibody
    • CXCR2_HUMAN antibody
    • GRO/MGSA receptor antibody
    • High affinity interleukin-8 receptor B antibody
    • IL 8 receptor type 2 antibody
    • IL 8R B antibody
    • IL-8 receptor type 2 antibody
    • IL-8R B antibody
    • IL8 RB antibody
    • IL8 receptor type 2 antibody
    • IL8R B antibody
    • IL8R2 antibody
    • IL8RA antibody
    • Interleukin 8 Receptor B antibody
    • Interleukin 8 receptor, beta antibody
    • Interleukin 8 receptor, type 2 antibody
    see all

Anti-CXCR2 antibody 画像

  • Anti-CXCR2 antibody (ab65968) at 2 µg/ml + Human colon carcinoma lysate

    Predicted band size : 41 kDa
    Observed band size : 41 kDa
  • IHC image of ab65968 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CXCR2 antibody (ab65968) 使用論文

ab65968 has not yet been referenced specifically in any publications.

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Thank you for your call today and for letting us know about the trouble with ab14935. As we discussed, I am sending a free of charge vial of ab65968 on the order ***, which should arrive tomorrow. Please keep me updated about the results using this rep...

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (human colon tumor cell-HCT116)
Loading amount 40 µg
Specification human colon tumor cell-HCT116
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

投稿 Sep 16 2011

Thank you for submitting an Abreview for ab25968. We greatly appreciate your feedback and strive to publish both negative and positive reviews, rewarding each with Abpoints. Before we publish negative Abreviews our Scientific Support team reviews e...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"