製品の概要

  • 製品名Anti-CXCR2 antibody
    CXCR2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CXCR2
  • アプリケーション適用あり: WB, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CXCR2.

    (Peptide available as ab24280.)

  • ポジティブ・コントロール
    • In Western Blot, ab21641 gave a positive signal in HL60 whole cell lysate and in human spleen tissue lysate. In IF, this antibody gave a positive signal in HeLa, HepG2 and MCF7 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab21641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF Use a concentration of 5 µg/ml.
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2.
    • 配列類似性Belongs to the G-protein coupled receptor 1 family.
    • 翻訳後修飾Phosphorylated upon ligand binding; which is required for desensitization.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C-X-C chemokine receptor type 2 antibody
      • CD 182 antibody
      • CD182 antibody
      • CD182 antigen antibody
      • CDw128b antibody
      • Chemokine (CXC) receptor 2 antibody
      • CMKAR2 antibody
      • CXC-R2 antibody
      • CXCR 2 antibody
      • CXCR-2 antibody
      • CXCR2 antibody
      • CXCR2_HUMAN antibody
      • GRO/MGSA receptor antibody
      • High affinity interleukin-8 receptor B antibody
      • IL 8 receptor type 2 antibody
      • IL 8R B antibody
      • IL-8 receptor type 2 antibody
      • IL-8R B antibody
      • IL8 RB antibody
      • IL8 receptor type 2 antibody
      • IL8R B antibody
      • IL8R2 antibody
      • IL8RA antibody
      • Interleukin 8 Receptor B antibody
      • Interleukin 8 receptor, beta antibody
      • Interleukin 8 receptor, type 2 antibody
      see all

    Anti-CXCR2 antibody 画像

    • ICC/IF image of ab21641 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21641, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.
    • All lanes : Anti-CXCR2 antibody (ab21641) at 1 µg/ml

      Lane 1 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Predicted band size : 41 kDa
      Observed band size : 41 kDa
      Additional bands at : 15 kDa,60 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 12 minutes

    Anti-CXCR2 antibody (ab21641) 使用論文

    ab21641 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab21641.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"