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Our Abpromise guarantee covers the use of ab14935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).Can be blocked with CXCR2 peptide (ab209353).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration.|
WB of CXCR2-201AP and PC-CXCR2. Antibody dilution 1:500 in Diluobuffer
ab14935 staining CXCR2 in Mouse spleen cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour. An Alexa Fluor® 555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.