製品の概要

  • 製品名Anti-CX3CR1 antibody
    CX3CR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CX3CR1
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide, corresponding to amino acids 175-189 of Human CX3CR 1.

  • ポジティブ・コントロール
    • THP-1 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab8020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.
WB 1/500. Predicted molecular weight: 50 kDa.Can be blocked with CX3CR1 peptide (ab8124).

ターゲット情報

  • 機能Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
  • 組織特異性Expressed in lymphoid and neural tissues.
  • 配列類似性Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta chemokine receptor-like 1 antibody
    • C X3 C CKR 1 antibody
    • C-X3-C CKR-1 antibody
    • CCRL1 antibody
    • Chemokine C X3 C motif receptor 1 antibody
    • CMK BRL 1 antibody
    • CMK-BRL-1 antibody
    • CMK-BRL1 antibody
    • CMKBLR1 antibody
    • CMKDR1 antibody
    • CX3C chemokine receptor 1 antibody
    • CX3C CKR1 antibody
    • CX3C1_HUMAN antibody
    • CX3CR1 antibody
    • Fractalkine receptor antibody
    • G-protein coupled receptor 13 antibody
    • GPR13 antibody
    • GPRV28 antibody
    • V28 antibody
    see all

Anti-CX3CR1 antibody 画像

  • All lanes : Anti-CX3CR1 antibody (ab8020) at 1/500 dilution

    Lane 1 : THP-1 cell lysate with absence of blocking peptide
    Lane 2 : THP-1 cell lysate with presence of blocking peptide


    Predicted band size : 50 kDa
    Observed band size : 50 kDa
  • ab8020 at 10µg/ml staining CX3CR1 in human spleen cells by IHC

  • Immunofluorescence of CX3CR1 in THP1 cells using ab8020 at 20 ug/ml.

Anti-CX3CR1 antibody (ab8020) 使用論文

This product has been referenced in:
  • Szukiewicz D  et al. CX3CL1 (fractalkine) and TNFa production by perfused human placental lobules under normoxic and hypoxic conditions in vitro: the importance of CX3CR1 signaling. Inflamm Res 63:179-89 (2014). IHC-P ; Human . Read more (PubMed: 24270813) »
  • Rooper L  et al. Fractalkine receptor is expressed in mature ovarian teratomas and required for epidermal lineage differentiation. J Ovarian Res 6:57 (2013). Read more (PubMed: 23958497) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (brain tissue)
Permeabilization Yes - 0.5% triton
Specification brain tissue
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative Formaldehyde
Username

Dr. Ali Ertürk

Verified customer

投稿 Oct 24 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (Brain slice - CA1 hippocampal section)
Permeabilization Yes - with blocking
Specification Brain slice - CA1 hippocampal section
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 13 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 80 µg
Specification Adult mouse brain. LANE NUMBER 2 IN THE FIGURE.
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 02 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 30 2015

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (gel 10%)
Sample Mouse Cell lysate - whole cell (Epithelial ovarian cancer cell line)
Specification Epithelial ovarian cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Hilal Gurler

Verified customer

投稿 Jun 18 2013

Thank you for your enquiry. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab7201. Also, ab8020 has not yet been tested for application in IHC-frozen sections (the antibody has only so far been tested in Wester...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"