製品の概要
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製品名Anti-CX3CR1 antibody
CX3CR1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to CX3CR1
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由来種Rabbit
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アプリケーション適用あり: IP, WB, IHC-P, ICC/IFmore details
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種交差性交差種: Human
交差が予測される動物種: Cow, Chimpanzee, Rhesus monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human CX3CR1.
(Peptide available as ab64633.)
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ポジティブ･コントロール
- This antibody gave a positive signal in the following Human Lysates: HeLa Whole Cell, SW480 Whole Cell, Human Small Intestine Tissue, JEG-3 Whole Cell, Y79 Whole Cell, HepG2 Whole Cell, Caco 2 Whole Cell
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab51668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 40,50 kDa (predicted molecular weight: 40 kDa).Can be blocked with CX3CR1 peptide (ab8125). | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
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機能Receptor for the CX3C chemokine fractalkine and mediates both its adhesive and migratory functions. Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1.
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組織特異性Expressed in lymphoid and neural tissues.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 100124525 Cow
- Entrez Gene: 1524 Human
- Omim: 601470 Human
- SwissProt: A6QNL7 Cow
- SwissProt: P49238 Human
- Unigene: 78913 Human
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別名
- Beta chemokine receptor-like 1 antibody
- C X3 C CKR 1 antibody
- C-X3-C CKR-1 antibody
see all
画像
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Immunoprecipitation - Anti-CX3CR1 antibody (ab51668)CX3CR1 was immunoprecipitated using 0.5mg JEG3 whole cell extract, 5µg of Rabbit polyclonal to CX3CR1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, JEG3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab51668.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 40kDa; CX3CR1
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Western blot - Anti-CX3CR1 antibody (ab51668)All lanes : Anti-CX3CR1 antibody (ab51668) at 1 µg
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :SW480 whole cell lysate (ab3957)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 7 :Caco 2 whole cell lysate (ab3950)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40,50 kDa (why is the actual band size different from the predicted?)
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
CX3CR1 has a predicted MW of 40 kDa and has 3 known isoforms (SwissProt). The 50 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to CX3CR1. -
Immunocytochemistry/ Immunofluorescence - Anti-CX3CR1 antibody (ab51668)ICC/IF image of ab51668 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51668, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
参考文献
ab51668 has not yet been referenced specifically in any publications.
レビューと Q&A
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