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Our Abpromise guarantee covers the use of ab25088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22387113|
|IHC-Fr||1/100 - 1/500.|
|IHC-P||Use a concentration of 4 µg/ml.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 42 kDa.|
The gel running conditions were denaturing.
The membrane was blocked with 10% milk for 30 minutes at RT and the primary antibody was incubated with the membrane for 16 hours at 4°C
ICC/IF image of ab25088 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25088, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"