The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
組織特異性Isoform 1 is highly expressed in adult and fetal heart, skeletal muscle, and at a much lower levels in adipose tissue and in aorta. Undetectable in other tissues. Isoform 2 and isoform 3 are poorly detected in heart.
配列類似性Belongs to the small heat shock protein (HSP20) family.
細胞内局在Cytoplasm. Nucleus. Nucleus > Cajal body. Resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.