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Recombinant fragment (proprietary-tag) corresponding to Human CUTL1 aa 521-621.
Our Abpromise guarantee covers the use of ab54583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry analysis of ab54583 antibody at 5 ug/ml with formalin-fixed, paraffin-embedded Human Spleen
Detection limit of ab54583 is approximately 0.03ng/ml as a capture antibody
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.