Anti-Protein CASP 抗体 [2A10] - BSA and Azide free (ab54583)
Key features and details
- Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-Protein CASP antibody [2A10] - BSA and Azide free
Protein CASP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2A10] to Protein CASP - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, Sandwich ELISA, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment (GST-tag) corresponding to Human Protein CASP aa 500-650. NP_001904.2
Database link: Q13948 -
ポジティブ・コントロール
- ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: Protein CASP transfected HEK-293T cell lysate. Recombinant Protein CASP protein.
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特記事項
This product was changed from ascites to tissue culture supernatant on 13th Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
2A10 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab54583の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa.
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ICC/IF | (3) |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
|
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 77.5 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May be involved in intra-Golgi retrograde transport. -
配列類似性
Belongs to the CASP family. -
細胞内局在
Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1523 Human
- SwissProt: Q13948 Human
- Unigene: 191482 Human
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別名
- CASP_HUMAN antibody
- CUX1 antibody
- Protein CASP antibody
画像
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Formalin-fixed, paraffin-embedded human spleen tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Protein CASP (green) using ab54583 at 10 µg/ml in ICC/IF.
This image was generated using the ascites version of the product.
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Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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All lanes : Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml
Lane 1 : Protein CASP transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 77.5 kDaThis image was generated using the ascites version of the product.
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Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for Protein CASP with ab54583 at 5 µg/ml in immunohistochemical analysis.
This image was generated using the ascites version of the product.
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Anti-Protein CASP antibody [2A10] - BSA and Azide free (ab54583) at 5 µg/ml + Tagged recombinant Protein CASP protein
Predicted band size: 77.5 kDaThis antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Expected MW 38 kDa.
This image was generated using the ascites version of the product.
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Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (22)
ab54583 は 22 報の論文で使用されています。
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Schilders KAA et al. Identification of SOX2 Interacting Proteins in the Developing Mouse Lung With Potential Implications for Congenital Diaphragmatic Hernia. Front Pediatr 10:881287 (2022). PubMed: 35615634
- Rosebrock D et al. Enhanced cortical neural stem cell identity through short SMAD and WNT inhibition in human cerebral organoids facilitates emergence of outer radial glial cells. Nat Cell Biol 24:981-995 (2022). PubMed: 35697781
- Ye T et al. Efficient manipulation of gene dosage in human iPSCs using CRISPR/Cas9 nickases. Commun Biol 4:195 (2021). PubMed: 33580208
- Pozo-Rodrigálvarez A et al. Hyperactive Behavior and Altered Brain Morphology in Adult Complement C3a Receptor Deficient Mice. Front Immunol 12:604812 (2021). PubMed: 33692783