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Our Abpromise guarantee covers the use of ab54583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 25355226|
|ICC/IF||Use a concentration of 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CUTL1 (green) using ab54583 at 10 µg/ml in ICC/IF.
Formalin-fixed, paraffin-embedded human spleen tissue stained for CUTL1 with ab54583 at 5 µg/ml in immunohistochemical analysis.
Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for CUTL1 with ab54583 at 5 µg/ml in immunohistochemical analysis.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Expected MW 38 kDa.
Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody