製品の概要

  • 製品名
    Anti-CUTL1 antibody [2A10]
    CUTL1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A10] to CUTL1
  • アプリケーション
    適用あり: IHC-P, ICC, WB, IHC-FoFr, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment (GST-tag) corresponding to Human CUTL1 aa 521-621. NP_001904.2
    Sequence:

    AENRLAQHTLQALQSELDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELR YSSQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTI


    Database link: Q13948

  • ポジティブ・コントロール
    • ICC/IF: HeLa cells. IHC-P: Human spleen tissue; human malignant lymphoma, diffuse large B tissue. Flow Cytometry: MCF7 cells. WB: CUTL1 transfected HEK-293T cell lysate. Recombinant CUTL1 protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml.
IHC-FoFr Use at an assay dependent concentration. PubMed: 25355226
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    Belongs to the CASP family. May be involved in intra-Golgi retrograde transport. Homodimer; disulfide-linked. Interacts with GOLGA5.
  • 細胞内局在
    Golgi apparatus membrane; Single-pass type IV membrane protein
  • 参照データベース
  • 別名
    • CASP antibody
    • CCAAT displacement protein antibody
    • CDP antibody
    • CDP/Cut antibody
    • CDP1 antibody
    • Clox antibody
    • COY1 antibody
    • Cut homeobox antibody
    • Cut homolog antibody
    • Cut like 1 antibody
    • Cut like homeobox 1 antibody
    • CUTL1 antibody
    • CUX antibody
    • Cux/CDP antibody
    • CUX1 antibody
    • Golgi integral membrane protein 6 antibody
    • GOLIM6 antibody
    • Homeobox protein cux 1 antibody
    • Nbla10317 antibody
    • p100 antibody
    • p110 antibody
    • p200 antibody
    • p75 antibody
    • Protein CASP antibody
    • Putative protein product of Nbla10317 antibody
    see all

画像

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for CUTL1 (green) using ab54583 at 10 µg/ml in ICC/IF.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for CUTL1 with ab54583 at 5 µg/ml in immunohistochemical analysis.

     

  • Overlay histogram showing MCF7 (human breast adenocarcinoma cell line) cells stained with ab54583 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3 M glycine to block non-specific protein-protein interactions followed by the antibody (ab54583, 1 µg/1 x 106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1 x 106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • All lanes : Anti-CUTL1 antibody [2A10] (ab54583) at 5 µg/ml

    Lane 1 : CUTL1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
  • Formalin-fixed, paraffin-embedded human malignant lymphoma, diffuse large B tissue stained for CUTL1 with ab54583 at 5 µg/ml in immunohistochemical analysis.

  • Anti-CUTL1 antibody [2A10] (ab54583) at 5 µg/ml + tagged recombinant CUTL1 protein

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Expected MW 38 kDa.

  • Detection limit of ab54583 is approximately 0.03 ng/ml as a capture antibody

参考文献

This product has been referenced in:
  • Edri R  et al. Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors. Nat Commun 6:6500 (2015). Other ; Human . Read more (PubMed: 25799239) »
  • Muratore CR  et al. The familial Alzheimer's disease APPV717I mutation alters APP processing and Tau expression in iPSC-derived neurons. Hum Mol Genet N/A:N/A (2014). WB ; Human . Read more (PubMed: 24524897) »

See all 9 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (brain)
Permeabilization
Yes - 0.3% triton
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 in PBS
Blocking step
10% normal serum, 1% BSA in PBST as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 May 02 2013

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain, placenta)
Specification
brain, placenta
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 20 min, 100C
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Nov 09 2011

Thank you for contacting us and sorry for the delay in getting back to you. We have a number of collaborators which produce antibodies for us as well as in house production of antibodies. The 5 antibodies that we have against CUTL1 have all been sou...

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy)
Loading amount
500000 cells
Specification
MDA-MB-231, HT-1080, NIH3T3, D283Med, Daoy
Gel Running Conditions
Reduced Denaturing (8%gel)
Blocking step
3% non fat milk/ 2% BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Ms. Sabine Topka

Verified customer

投稿 Oct 07 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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