製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cullin 7
  • 特異性
    Ab34841 is recognizes Cullin 7, but cross reactivity with other human cullins may occur.
  • アプリケーション
    適用あり: IP, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    RSRGPVYASCTATQSFSTFR

    conjugated to KLH, corresponding to C terminal amino acids 1679 - 1698 of Human Cullin 7.

  • ポジティブ・コントロール
    • IP: Protein from cell lysates (using HeLa, NIH-3T3, and others). Most cell lines expressing Cullin 7 can be used as a positive control.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: Whole serum
  • Concentration information loading...
  • 特記事項(精製)
    Ab34841 is a monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
ELISA
WB
  • 追加情報
    ELISA: 1/2000 - 1/10000.
    IP: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 191 kDa (predicted molecular weight: 191 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation (By similarity). Seems not to promote polyubiquitination and proteasomal degradation of TP53. In vitro, complexes of CUL7 with either CUL9 or FBXW8 or TP53 contain E3 ubiquitin-protein ligase activity.
    • 組織特異性
      Highly expressed in fetal kidney and adult skeletal muscle. Also abundant in fetal brain, as well as in adult pancreas, kidney, placenta and heart. Detected in trophoblasts, lymphoblasts, osteoblasts, chondrocytes and skin fibroblasts.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 関連疾患
      Defects in CUL7 are the cause of 3M syndrome type 1 (3M1) [MIM:273750]. An autosomal recessive disorder characterized by severe pre- and postnatal growth retardation, facial dysmorphism, large head circumference, and normal intelligence and endocrine function. Skeletal changes include long slender tubular bones and tall vertebral bodies.
    • 配列類似性
      Belongs to the cullin family.
      Contains 1 DOC domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CUL-7 antibody
      • CUL7 antibody
      • CUL7_HUMAN antibody
      • Cullin-7 antibody
      • dJ20C7.5 antibody
      • KIAA0076 antibody
      see all

    参考文献

    ab34841 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    投稿 May 12 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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