製品の概要

  • 製品名Anti-Cullin 3 antibody
    Cullin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Cullin 3
  • アプリケーション適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide, corresponding to amino acids 718-768 of Human Cullin 3 (NP_003581.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate NIH3T3 whole cell lysate 293T whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the BCR complex depends on the BTB domain-containing protein as the susbstrate recognition component. BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, H2AFY and DAXX, and probably GLI2 or GLI3. BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. Involved in ubiquitination of cyclin E and of cyclin D1 (in vitro) thus involved in regulation of G1/S transition.
    • 組織特異性Widely expressed.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the cullin family.
    • 翻訳後修飾Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • 細胞内局在Nucleus. Golgi apparatus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CUL 3 antibody
      • Cul-3 antibody
      • cul3 antibody
      • CUL3_HUMAN antibody
      • Cullin-3 antibody
      • Cullin3 antibody
      • KIAA0617 antibody
      • PHA2E antibody
      see all

    Anti-Cullin 3 antibody 画像

    • All lanes : Anti-Cullin 3 antibody (ab72187) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg
      Lane 5 : NIH3T3 whole cell lysate at 50 µg

      Developed using the ECL technique

      Predicted band size : 89 kDa
      Observed band size : 89 kDa


      Exposure time : 3 minutes

    Anti-Cullin 3 antibody (ab72187) 使用論文

    This product has been referenced in:
    • Gastaldello S  et al. Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells. PLoS Pathog 9:e1003664 (2013). Human . Read more (PubMed: 24130483) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"