製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Cullin 2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide from the C-terminus of human Cullin 2

  • ポジティブ・コントロール
    • Raji cells. Tonsil.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74280 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報
    IHC-P: 1/200 for 10 min at RT. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 mins, followed by cooling at RT for 20 mins.
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 87 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 配列類似性
      Belongs to the cullin family.
    • 翻訳後修飾
      CBC(VHL) complex formation seems to promote neddylation. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Cul2 antibody
      • CUL 2 antibody
      • CUL-2 antibody
      • CUL2 antibody
      • CUL2_HUMAN antibody
      • cullin 2 antibody
      • cullin homolog 2 antibody
      • Cullin-2 antibody
      • MGC131970 antibody
      see all

    画像

    • Staining of formalin-fixed, paraffin-embedded human tonsil tissue using 1/200 ab74280.

    参考文献

    ab74280 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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