製品の概要

  • 製品名Anti-Cullin 1 antibody [EPR3102Y]
    Cullin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3102Y] to Cullin 1
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human)

  • ポジティブ・コントロール
    • 293T, A549 and T47D cell lysates; HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab75812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 90 kDa.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
    • 組織特異性Expressed in lung fibroblasts.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Belongs to the cullin family.
    • 翻訳後修飾Neddylated; which enhances the ubiquitination activity of SCF. Deneddylated via its interaction with the COP9 signalosome (CSN) complex. Deneddylated by Epstein-Barr virus BPLF1 leading to a S-phase-like environment that is required for efficient replication of the viral genome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CUL 1 antibody
      • CUL-1 antibody
      • CUL1 antibody
      • CUL1_HUMAN antibody
      • Cullin-1 antibody
      • Cullin1 antibody
      • MGC149834 antibody
      • MGC149835 antibody
      see all

    Anti-Cullin 1 antibody [EPR3102Y] 画像

    • ab75812 at 1/100 dilution staining Cullin 1 in HeLa cells by Immunofluorescence.
    • All lanes : Anti-Cullin 1 antibody [EPR3102Y] (ab75812) at 1/2000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : T47D cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 90 kDa
      Observed band size : 90 kDa

    Anti-Cullin 1 antibody [EPR3102Y] (ab75812) 使用論文

    This product has been referenced in:
    • Bruckert WM  et al. Rapid nutritional remodeling of the host cell upon attachment of Legionella pneumophila. Infect Immun 82:72-82 (2014). Read more (PubMed: 24126522) »
    • Kainulainen M  et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . Read more (PubMed: 24403578) »

    See all 3 Publications for this product

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