製品の概要

  • 製品名
    Anti-CUEDC1 antibody
  • 製品の詳細
    Rabbit polyclonal to CUEDC1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: RDFLLALERD RLKYESQKSK SSSVAVGNDF GFSSPVPGTG DANPAVSEDA, corresponding to internal sequence amino acids 253-302 of Human CUEDC1

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab58696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

ターゲット情報

画像

  • Anti-CUEDC1 antibody (ab58696) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with skimmed milk in PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab58696 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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