The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human CTRP4 peptide (ab39741).
Use a concentration of 10 µg/ml.
Use a concentration of 20 µg/ml.
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. Multiple isoforms of mouse CTRP4 have been reported.
C1q and tumor necrosis factor related protein 4 antibody
Complement C1q tumor necrosis factor related protein antibody
Western blot - CTRP4 antibody (ab36871)
Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml
Lane 1 : Rat brain cell lysate Lane 2 : Rat brain cell lysate Lane 3 : Rat brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size : 37 kDa Observed band size : 35 kDa (why is the actual band size different from the predicted?) Lanes read from left to right 1, 2 and 3.
These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size.
Byerly MS et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem289:4055-69 (2014).
Read more (PubMed: 24366864) »