製品の概要

  • 製品名Anti-CTLA4 antibody [BNI3]
    CTLA4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BNI3] to CTLA4
  • アプリケーション適用あり: ICC/IF, Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human CTLA-4/human IgG heavy chain fusion protein (CTLA-4/Ig).

  • ポジティブ・コントロール
    • ICC/FACS: B cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 - 50 µg/ml.
Flow Cyt Use at an assay dependent concentration.

Use 2µg for 5x 105 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use a concentration of 5 - 50 µg/ml.

ターゲット情報

  • 機能Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
  • 組織特異性Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
  • 関連疾患Genetic variation in CTLA4 influences susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    Note=Genetic variations in CTLA4 may influence susceptibility to Graves disease, an autoimmune disorder associated with overactivity of the thyroid gland and hyperthyroidism.
    Genetic variation in CTLA4 is the cause of susceptibility to diabetes mellitus insulin-dependent type 12 (IDDM12) [MIM:601388]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
    Genetic variation in CTLA4 is the cause of susceptibility to celiac disease type 3 (CELIAC3) [MIM:609755]. It is a multifactorial disorder of the small intestine that is influenced by both environmental and genetic factors. It is characterized by malabsorption resulting from inflammatory injury to the mucosa of the small intestine after the ingestion of wheat gluten or related rye and barley proteins. In its classic form, celiac disease is characterized in children by malabsorption and failure to thrive.
  • 配列類似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 翻訳後修飾N-glycosylation is important for dimerization.
    Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
  • 細胞内局在Cell membrane. Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation and.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALPS5 antibody
    • CD 152 antibody
    • CD antibody
    • CD152 antibody
    • CD152 antigen antibody
    • CD152 isoform antibody
    • Celiac disease 3 antibody
    • CELIAC3 antibody
    • CTLA 4 antibody
    • CTLA-4 antibody
    • CTLA4 antibody
    • CTLA4_HUMAN antibody
    • Cytotoxic T cell associated 4 antibody
    • Cytotoxic T lymphocyte antigen 4 antibody
    • Cytotoxic T lymphocyte associated 4 antibody
    • Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody
    • Cytotoxic T lymphocyte associated antigen 4 antibody
    • Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody
    • Cytotoxic T lymphocyte associated protein 4 antibody
    • Cytotoxic T lymphocyte associated serine esterase 4 antibody
    • Cytotoxic T lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte protein 4 antibody
    • Cytotoxic T-lymphocyte-associated antigen 4 antibody
    • GRD4 antibody
    • GSE antibody
    • ICOS antibody
    • IDDM12 antibody
    • insulin-dependent diabetes mellitus 12 antibody
    • Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody
    • OTTHUMP00000216623 antibody
    see all

Anti-CTLA4 antibody [BNI3] (ab19792) 使用論文

This product has been referenced in:
  • Mittal AK  et al. Role of CTLA4 in the proliferation and survival of chronic lymphocytic leukemia. PLoS One 8:e70352 (2013). WB ; Human . Read more (PubMed: 23936412) »
  • Suárez-Fariñas M  et al. Expanding the Psoriasis Disease Profile: Interrogation of the Skin and Serum of Patients with Moderate-to-Severe Psoriasis. J Invest Dermatol : (2012). IHC-Fr ; Human . Read more (PubMed: 22763790) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Lymph node)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Both Tris-EDTA and Citrate-Sodium
Permeabilization No
Specification Lymph node
Blocking step Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 12 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Renal cell carcinoma, melanoma, T-cells)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate, Tris-EDTA
Permeabilization No
Specification Renal cell carcinoma, melanoma, T-cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"