製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Ctip2
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).

  • ポジティブ・コントロール
    • Nuclear extract from Jurkat cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/15000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa).
IP Use at 1-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • 組織特異性
    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • 配列類似性
    Contains 6 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes: 1mg nuclear extract from Jurkat cells analysed on a blot using ab70453 at 0.5µg/ml.
    Lane 1: an alternate Ctip2 antibody used at 1 µg/mg lysate for IP.
    Lane 2: The same alternate antibody used at 10 µg/mg lysate for IP.
    Lane 3: ab70453 used at 1 µg/mg lysate for IP.
    Lane 4: ab70453 at 10 µg/mg lysate for IP.
    Normal rabbit IgG was used as a control for immunoprecipitation reactions.
  • All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml

    Lane 1 : Jurkat cell nuclear extract at 5 µg
    Lane 2 : Jurkat cell nuclear extract at 50 µg


    Predicted band size : 100 kDa
    Observed band size : 120-130 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Ctip2 antibody (ab70453) at 0.2 µg/ml

    Lane 1 : L1210 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26.WT whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 100 kDa


    Exposure time : 3 minutes

参考文献

ab70453 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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