Anti-Ctip2 抗体 [25B6] - ChIP Grade (ab18465)

製品の概要

  • 製品名
    Anti-Ctip2 antibody [25B6] - ChIP Grade
    Ctip2 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [25B6] to Ctip2 - ChIP Grade
  • 由来種
    Rat
  • 特異性
    Detects 2 bands representing Ctip2 at about 120kD. Ctip2 is highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • アプリケーション
    適用あり: ICC/IF, IHC-FoFr, WB, IP, IHC-P, IHC-Fr, ChIP, IHC-FrFl, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Guinea pig, Human, Zebrafish, Cynomolgus monkey
  • 免疫原

    Fusion protein corresponding to Human Ctip2.

  • エピトープ
    Between amino acids 1-150 of CTIP2.
  • ポジティブ・コントロール
    • WB: nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody IHC: adult mouse hippocampus, Adult mouse spinal cord ICC: Neonatal mouse hippocampal cultured neurons
  • 特記事項

    Hybridoma produced by fusion of a rat lymphocyte and mouse myeloma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18523013
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 95 kDa).
IP Use at an assay dependent concentration.
IHC-P 1/500.
IHC-Fr 1/500.
ChIP Use at an assay dependent concentration.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element.
  • 組織特異性
    Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma.
  • 配列類似性
    Contains 6 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATL1 alpha antibody
    • ATL1 antibody
    • ATL1 beta antibody
    • ATL1 gamma antibody
    • ATL1-delta antibody
    • B cell CLL/lymphoma 11B/T cell receptor delta constant region fusion protein antibody
    • B cell lymphoma/leukemia 11B antibody
    • B-cell CLL/lymphoma 11B (zinc finger protein) antibody
    • B-cell CLL/lymphoma 11B antibody
    • B-cell lymphoma/leukemia 11B antibody
    • BC11B_HUMAN antibody
    • BCL-11B antibody
    • Bcl11b antibody
    • BCL11B/TRDC fusion antibody
    • COUP TF interacting protein 2 antibody
    • COUP-TF interacting protein 2 antibody
    • COUP-TF-interacting protein 2 antibody
    • Ctip 2 antibody
    • CTIP-2 antibody
    • CTIP2 antibody
    • hRIT1 alpha antibody
    • hRit1 antibody
    • Radiation induced tumor suppressor gene 1 antibody
    • Radiation induced tumor suppressor gene 1 protein antibody
    • Radiation-induced tumor suppressor gene 1 protein antibody
    • Rit 1 antibody
    • Rit1 antibody
    • zinc finger protein hRit1 alpha antibody
    • ZNF856B antibody
    see all

画像

  • Neonatal Mouse Hippocampal Neurons (Harvested at P1, grown 5d in culture on glial cell feeder layer).

    Red is beta tubulin staining.
    Green is ab18465.

  • ab18465 staining Ctip2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG (1/1000) was used as secondary antibody. Staining was improved with citrate antigen retrieval.

    See Abreview

  • Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation, green is ab18465 alone and red is Ctip1 antibody alone.
  • Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

    Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

    Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).
  • ab18465 staining Ctip2 in Guinea Pig Brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in blocking buffer) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rat IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry using ab18465 on Adult Mouse Hippocampus.
  • All lanes : Anti-Ctip2 antibody [25B6] - ChIP Grade (ab18465) at 1/500 dilution

    Lanes 1-2 : Mouse brain tissue lysate at 1.5 µg
    Lane 3 : Mouse brain tissue lysate at 3 µg

    Secondary
    All lanes : IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Observed band size: 100,110 kDa (why is the actual band size different from the predicted?)


    Exposure time: 10 minutes

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Wang CY  et al. Function of B-Cell CLL/Lymphoma 11B in Glial Progenitor Proliferation and Oligodendrocyte Maturation. Front Mol Neurosci 11:4 (2018). WB ; Rat . Read more (PubMed: 29416501) »
  • Thion MS  et al. Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell 172:500-516.e16 (2018). Read more (PubMed: 29275859) »

See all 221 Publications for this product

レビューと Q&A

Thanks for passing along the protocol details. They all seem quite reasonable.

If I could I'd just like to ask a few additional questions:

1. Are you still using same vial of antibody?

2. Have you stored the antibody in al...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Tonsil
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (e15 brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
e15 brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Apr 04 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human cerebral organoids)
Specification
Human cerebral organoids
Blocking step
Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 4°C
Fixative
Paraformaldehyde
Username

Radhika Menon

Verified customer

投稿 Jul 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 10 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain, striatum)
Specification
Brain, striatum
Username

Abcam user community

Verified customer

投稿 May 23 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 04 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 May 04 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Triton x 100: 30 min
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 04 2016

1-10 of 47 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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