Anti-Ctip1 抗体 [18B12DE6] - ChIP Grade (ab19489)

製品の概要

  • 製品名
    Anti-Ctip1 antibody [18B12DE6] - ChIP Grade
    Ctip1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [18B12DE6] to Ctip1 - ChIP Grade
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, ICC, IP, Flow Cyt, ChIP, IHC-FrFlmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A cocktail of BCL11A fusion proteins.

  • エピトープ
    Epitope is in carboxyl terminus of CTIP1/Bcl11a (aa's 434-776).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 91 kDa.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 20542454
IHC-FrFl Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1.
  • 組織特異性
    Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development.
  • 関連疾患
    Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification.
  • 配列類似性
    Contains 6 C2H2-type zinc fingers.
  • 翻訳後修飾
    Sumoylated by SUMO1.
  • 細胞内局在
    Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810047E18Rik antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) antibody
    • B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
    • B-cell CLL/lymphoma 11A antibody
    • B-cell lymphoma/leukemia 11A antibody
    • BC11A_HUMAN antibody
    • BCL-11A antibody
    • BCL11A antibody
    • BCL11A B cell CLL/lymphoma 11A (zinc finger protein) isoform 1 antibody
    • BCL11A L antibody
    • BCL11A S antibody
    • BCL11A XL antibody
    • BCL11a-M antibody
    • BCL11AL antibody
    • BCL11AS antibody
    • BCL11AXL antibody
    • C2H2 type zinc finger protein antibody
    • COUP TF interacting protein 1 antibody
    • COUP-TF-interacting protein 1 antibody
    • CTIP1 antibody
    • CTIP1, mouse, homolog of antibody
    • D930021L15Rik antibody
    • Ecotropic viral integration site 9 antibody
    • Ecotropic viral integration site 9 homolog antibody
    • Ecotropic viral integration site 9 protein antibody
    • Ecotropic viral integration site 9 protein homolog antibody
    • EVI-9 antibody
    • Evi9 antibody
    • Evi9, mouse, homolog of antibody
    • FLJ10173 antibody
    • FLJ34997 antibody
    • HBFQTL5 antibody
    • KIAA1809 antibody
    • mKIAA1809 antibody
    • OTTHUMP00000159788 antibody
    • OTTHUMP00000159789 antibody
    • OTTHUMP00000201250 antibody
    • OTTHUMP00000202084 antibody
    • Zinc finger protein 856 antibody
    • ZNF856 antibody
    see all

画像



  • Predicted band size : 91 kDa

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Empty (0 µg)
    Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Raji whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab19489 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab19489 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Empty knockout samples were subjected to SDS-PAGE. ab19489 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Human normal tonsil. Staining is localised to cytoplasm and nuclei. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Overlay histogram showing Ramos cells stained with ab19489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19489, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:
  • Chan CM  et al. A signature motif mediating selective interactions of BCL11A with the NR2E/F subfamily of orphan nuclear receptors. Nucleic Acids Res N/A:N/A (2013). ChIP . Read more (PubMed: 23975195) »
  • Lee ST  et al. A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network. Nucleic Acids Res 40:11339-51 (2012). IP ; Human . Read more (PubMed: 23074194) »

See all 5 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (mesenchymal stem cells)
Permeabilization
Yes - Triton 0.25%
Specification
mesenchymal stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 17 2016

Application
ChIP
Sample
Human Cell lysate - nuclear (Lymphoblastoid cell line)
Negative control
No antibody control for ChIP. Negative region for qPCR.
Specification
Lymphoblastoid cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: PFA 1%
Positive control
ChIP with anti-Pol2, anti-IRF4, anti-MEF2A, anti-RELA antibodies. The Possitive regions assessed by qPCR (IGLL5-Enh, LAPTM5-Enh) were validated using ENCODE data to be bound by studied TFs.
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投稿 Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
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投稿 Dec 23 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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