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Our Abpromise guarantee covers the use of ab19487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC (PFA fixed)||Use at an assay dependent concentration.|
|ChIP||Use at an assay dependent concentration. PubMed: 23576758|
|Flow Cyt||Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
The CTIP1 protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.
Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4ºC.