Anti-Ctip1/BCL-11A 抗体 [14B5] (ab19487)
Key features and details
- Mouse monoclonal [14B5] to Ctip1/BCL-11A
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Ctip1/BCL-11A antibody [14B5]
Ctip1/BCL-11A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [14B5] to Ctip1/BCL-11A -
由来種
Mouse -
特異性
Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.
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アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Fusion protein corresponding to Ctip1/BCL-11A.
Database link: Q9H165 -
エピトープ
Epitope is in core of CTIP1/Bcl11a (aa's 172-434). -
ポジティブ・コントロール
- WB: Raji whole cell lysate, Jurkat cell line, MEF cell line and HEK293T cell line. Flow Cyt: Ramos cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.50
Preservative: 0.02% Sodium azide
Constituent: HEPES -
Concentration information loading...
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精製度
Multi-step Chromatography -
ポリ/モノ
モノクローナル -
クローン名
14B5 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19487の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa). |
Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Functions as a myeloid and B-cell proto-oncogene. May play important roles in leukemogenesis and hematopoiesis. An essential factor in lymphopoiesis, is required for B-cell formation in fetal liver. May function as a modulator of the transcriptional repression activity of ARP1. -
組織特異性
Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development. -
関連疾患
Note=Chromosomal aberrations involving BCL11A may be a cause of lymphoid malignancies. Translocation t(2;14)(p13;q32.3) causes BCL11A deregulation and amplification. -
配列類似性
Contains 6 C2H2-type zinc fingers. -
翻訳後修飾
Sumoylated by SUMO1. -
細胞内局在
Cytoplasm. Nucleus. Associates with the nuclear body. Colocalizes with SUMO1 and SENP2 in nuclear speckles. - Information by UniProt
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参照データベース
- Entrez Gene: 53335 Human
- Entrez Gene: 14025 Mouse
- Omim: 606557 Human
- SwissProt: Q9H165 Human
- SwissProt: Q9QYE3 Mouse
- Unigene: 370549 Human
- Unigene: 441102 Mouse
- Unigene: 471050 Mouse
see all -
別名
- 2810047E18Rik antibody
- B cell CLL/lymphoma 11A (zinc finger protein) antibody
- B cell CLL/lymphoma 11A (zinc finger protein) isoform 2 antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Empty (0 µg)
Lane 3: BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 µg)
Lane 4: Raji whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab19487 observed at 91 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab19487 was shown to specifically react with BCL11A (Ctip1) in HAP1 wild type cells. No band was observed when BCL11A (Ctip1) knockout cells were examined. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. ab19487 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-Ctip1/BCL-11A antibody [14B5] (ab19487)
Lane 1 : C6 Cell Line
Lane 2 : MEF Cell Line
Lane 3 : HDFn Cell LIne
Lane 4 : Jurkat Cell Line
Lane 5 : HEK293T Cell Line
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDaThe CTIP1 / BCL-11A protein band is indicated at 92 kDa. This antibody also identified a 55 kDa protein band in Western blotting, principally in mouse fibroblasts (MEF), but this is unrelated to.
Blocking was performed in 5% milk (in PBS). The primary antibody was diluted in 1% milk (in PBS) and incubated overnight at 4ºC.
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Overlay histogram showing Ramos cells stained with ab19487 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19487, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) at 1 µg/ml + Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 37 kDa, 42 kDa, 62 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesThe 120 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Ctip1 / BCL-11A.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab19487 は 36 報の論文で使用されています。
- Qin K et al. Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nat Genet 54:874-884 (2022). PubMed: 35618846
- Wiegreffe C et al. Developmental cell death of cortical projection neurons is controlled by a Bcl11a/Bcl6-dependent pathway. EMBO Rep 23:e54104 (2022). PubMed: 35766181
- Mehta S et al. Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells. Cell Chem Biol 29:1273-1287.e8 (2022). PubMed: 35839780
- Angius A et al. Deciphering clinical significance of BCL11A isoforms and protein expression roles in triple-negative breast cancer subtype. J Cancer Res Clin Oncol N/A:N/A (2022). PubMed: 36030436
- Lan X et al. ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. Mol Cell 81:239-254.e8 (2021). PubMed: 33301730