製品の概要

  • 製品名Anti-CtIP antibody
    CtIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CtIP
  • アプリケーション適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human CtIP aa 525-575.
    Database link: Q99708

  • ポジティブ・コントロール
    • Whole cell lysate from MCF7 cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations.
    • 関連疾患Seckel syndrome 2
      Jawad syndrome
      Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986).
    • 配列類似性Belongs to the COM1/SAE2/CtIP family.
    • ドメインThe PXDLS motif binds to a cleft in CtBP proteins.
      The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage.
    • 翻訳後修飾Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
      Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
      Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057).
    • 細胞内局在Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604).
    • Information by UniProt
    • 参照データベース
    • 別名
      • COM1 antibody
      • COM1_HUMAN antibody
      • CtBP interacting protein antibody
      • CtBP-interacting protein antibody
      • CtIP antibody
      • DNA endonuclease RBBP8 antibody
      • JWDS antibody
      • RBBP-8 antibody
      • RBBP8 antibody
      • Retinoblastoma-binding protein 8 antibody
      • Retinoblastoma-interacting protein and myosin-like antibody
      • Rim antibody
      • SAE2 antibody
      • SCKL2 antibody
      • Sporulation in the absence of SPO11 protein 2 homolog antibody
      see all

    Anti-CtIP antibody 画像

    • CtIP was immunoprecipitated from 1 mg of MCF cells with ab70162 at 3 ug/mg.

      Detection:Chemiluminescence

      Exposure time:30 seconds

    Anti-CtIP antibody (ab70162) 使用論文

    ab70162 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab70162.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"