製品の概要

  • 製品名Anti-CTHRC1 antibody
    CTHRC1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CTHRC1
  • アプリケーション適用あり: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human CTHRC1.

    (Peptide available as ab101727.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Brain (Human) Tissue Lysate; Heart (Human) Tissue Lysate; Blood Vessel: artery (Human) Membrane Lysate; HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate; HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 26 kDa).
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-CTHRC1 antibody 画像

  • All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 minutes
  • Ab85739 staining CTHRC1 on E8.5 mouse embryo sections by immunohistochemistry. The tissue was paraformaldehyde fixed and permeabilized in PBST prior to blocking in NOVOCASTRA protein block for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 48 hour at 4°C. An Alexa Fluor 555 conjugated donkey anti-rabbit antibody, diluted 1/1000, was used as the secondary. Nuclear staining shown with DAPI.

    See Abreview

  • ICC/IF image of ab85739 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85739, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-CTHRC1 antibody (ab85739) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989)
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
    Lane 5 : Human heart tissue lysate - total protein (ab29431) with Human CTHRC1 peptide (ab101727) at 1 µg/ml
    Lane 6 : Human blood vessel: artery normal tissue lysate - membrane extract (ab28989) with Human CTHRC1 peptide (ab101727) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 26 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes
  • IHC image of CTHRC1 staining in human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85739, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-CTHRC1 antibody (ab85739) 使用論文

This product has been referenced in:
  • Qi C  et al. Preliminary observations regarding the expression of collagen triple helix repeat-containing 1 is an independent prognostic factor for Wilms' tumor. J Pediatr Surg 51:1501-6 (2016). WB, IHC-P ; Human . Read more (PubMed: 27230801) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (tail bud)
Loading amount 1 µg
Specification tail bud
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
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Abcam user community

Verified customer

投稿 Aug 22 2012

Thank you for contacting Abcam regarding ab85739.


This antibody is currently provided in a buffer containing BSA and Sodium Azide, but at concentrations that would not inhibit a typical conjugation reaction. Therefore, it is suitable for ...

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Thank you for contacting us and sorry for the delay in getting back to you.

I now have further information to share with you in regards to the anti-CTHRC1 antibody (ab85739). In order to try to validate the specificity of the antibody we have...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E8.5 mouse embryo sections)
Specification E8.5 mouse embryo sections
Fixative Paraformaldehyde
Permeabilization Yes - PBST (PBS/0.5%v/v TritonX100 (Sigma)
Blocking step NOVOCASTRA protein block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

Thank you for contacting us and making us aware of your concerns over the anti-CTHRC1 antibody (ab85739).

Unfortunately,wedo not have any furthertesting information than what is shown on the public datasheet for this antibody. We are currentl...

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Thank you for contacting Abcam Technical Team and I am very sorry to hear that you are having problems with this product.   I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"