製品の概要

  • 製品名Anti-CTGF antibody
    CTGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CTGF
  • 特異性This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.
  • アプリケーション適用あり: IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Rat, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human CTGF aa 150-250 conjugated to keyhole limpet haemocyanin.
    Database link: P29279
    (Peptide available as ab13741)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in recombinant human CTGF in WB; This antibody gave a positive signal in the following Formaldehyde fixed cell lines:HepG2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration. PubMed: 17028989
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/200.

ターゲット情報

  • 機能Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
  • 組織特異性Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.
  • 配列類似性Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.
  • 細胞内局在Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

Anti-CTGF antibody 画像

  • ab5097 staining CTGF in human heart tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in buffer and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/50 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.

    See Abreview

  • ab5097 staining CTGF in human bronchus tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and an antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab5097 at a 1/400 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit polyclonal.

    See Abreview

  • ICC/IF image of ab5097 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab5097 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab5097 staining CTGF in Pig skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS Tween 0.01% + donkey serum 1%) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Anti-CTGF antibody (ab5097) 使用論文

This product has been referenced in:
  • Meng YH  et al. Elevated expression of connective tissue growth factor, osteopontin and increased collagen content in human ascending thoracic aortic aneurysms. Vascular 22:20-7 (2014). IHC ; Human . Read more (PubMed: 23508392) »
  • Gillette M  et al. P190B RhoGAP overexpression in the developing mammary epithelium induces TGFß-dependent fibroblast activation. PLoS One 8:e65105 (2013). WB ; Mouse . Read more (PubMed: 23717689) »

See all 7 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Specification skin
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Pig Tissue sections (Ureter)
Specification Ureter
Fixative Paraformaldehyde
Permeabilization Yes - 0,2 % Trition/PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Ms. Beate Thode

Verified customer

投稿 Feb 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela, Cervix Cancer Cells)
Specification Hela, Cervix Cancer Cells
Fixative Paraformaldehyde
Permeabilization Yes - 0,2 Triton/PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1%
Username

Ms. Beate Thode

Verified customer

投稿 Feb 22 2013

Thank you for your reply and for your cooperation.

As agreed, I have issued a free of charge replacement with one unit of ab6328; the order number is ****.

To check the status of the order please contact our Customer Service team and ...

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En compensation de tous les problèmes rencontrés avec les anti-CTGF, j'aimerais vous offrir un avoir contre la commande originale du ab5097 ou un anticorps de remplacement de votre choix.

Merci de m'indiquer la résolution ...

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J'espère que vous allez bien.

J'aimerais vous informer que mes collègues du laboratoire ont effectué de nouveaux tests en Western Blot avec l'anti-CTGF référence ab5097.

Ils ont essayé diff&ea...

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Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

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Thank you for your message. We appreciate your valuable feedback in the Abreviews.

I can confirm that you will need to place your order using the testing discount code forthe free of charge antibody before 20th August.

I hope this...

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Merci beaucoup pour ce complément d'information.

Je vous contacterai de nouveau dès que j'aurai reçu les résultats du labo pour les anti-CTGF.

Je vous souhaite un très bon week-end.

Thank you very much for your email and the detailed information about your results.

I forwarded your data to our characterisation team who, since your last email, have started to investigate the problem. It has been decided to retest the produ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"