All lanes : Anti-CTBP2 antibody (ab113265) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 49 kDa
Exposure time : 3 minutes
Immunoprecipitation - CTBP2 antibody (ab113265)
Lane 1: 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab113265 at 6µg/mg lysate and 20% IP was loaded on gel. Lane 2: same, with a control IgG.
For WB ab113265 was used at 1µg/ml. Detection:
chemiluminescence with exposure times of 30 seconds.