製品の概要

  • 製品名Anti-CtBP1 (phospho S422) antibody
    CtBP1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CtBP1 (phospho S422)
  • 特異性Detects endogenous levels of CtBP1 only when phosphorylated at Serine 422.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from Human CtBP1 around the phosphorylation site of Serine 422 (A-P-SP-P-G).

  • ポジティブ・コントロール
    • WB: lysate from Jurkat cells pre-treated with TNF (20ng/ml, 30mins); IHC-P: human brain tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/5000.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Predicted molecular weight: 49 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity.
    • 配列類似性Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
    • 翻訳後修飾The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
      ADP-ribosylated; when cells are exposed to brefeldin A.
      Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.
    • 細胞内局在Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BARS antibody
      • brefeldin A- ribosylated substrate antibody
      • C terminal binding protein 1 antibody
      • C-terminal-binding protein 1 antibody
      • CTBP antibody
      • CtBP1 antibody
      • CTBP1_HUMAN antibody
      • MGC104684 antibody
      see all

    Anti-CtBP1 (phospho S422) antibody 画像

    • All lanes : Anti-CtBP1 (phospho S422) antibody (ab92577) at 1/500 dilution

      Lane 1 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 2 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins)
      Lane 3 : lysate from Jurkat cells treated with TNF (20ng/ml, 30mins) with immunizing phosphopeptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 49 kDa
    • Left panel: ab92577, at a 1/50 dilution, staining phosphorylated CtBP1 in formalin/ fixed, paraffin embedded Human tissue brain, using Immunohistochemistry.
      Right panel: same, after pre-incubation with the immunizing phosphopeptide.

    Anti-CtBP1 (phospho S422) antibody (ab92577) 使用論文

    ab92577 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab92577.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"