Anti-CtBP1 抗体 [EPR6800] (ab129181)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6800] to CtBP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CtBP1 antibody [EPR6800]
CtBP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6800] to CtBP1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human CtBP1 aa 350-450. The exact sequence is proprietary.
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ポジティブ・コントロール
- IHC-P: Human colon tissue. WB: SH-SY5Y, Raji, A549 and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells. Flow Cyt (intra): Raji cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 1.50 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6800 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129181の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/2000.
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WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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特記事項 |
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Flow Cyt (Intra)
1/2000. |
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
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機能
Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. -
配列類似性
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. -
翻訳後修飾
The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.
ADP-ribosylated; when cells are exposed to brefeldin A.
Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1487 Human
- Entrez Gene: 13016 Mouse
- Entrez Gene: 29382 Rat
- Omim: 602618 Human
- SwissProt: Q13363 Human
- SwissProt: O88712 Mouse
- SwissProt: Q9Z2F5 Rat
- Unigene: 208597 Human
see all -
別名
- BARS antibody
- brefeldin A- ribosylated substrate antibody
- C terminal binding protein 1 antibody
see all
画像
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All lanes : Anti-CtBP1 antibody [EPR6800] (ab129181) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Raji cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 48 kDa
Observed band size: 48 kDa -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling CtBP1 with ab129181 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
ab129181, at 1/100 dilution, staining CtBP1 in formalin-fixed, paraffin-embedded human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling CtBP1 (red) with ab129181 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab129181 は 11 報の論文で使用されています。
- Liang X et al. Angiotensin II Inhibits Adipogenic Differentiation and Promotes Mature Adipocyte Browning through the Corepressor CtBP1. Biomedicines 10:N/A (2022). PubMed: 36551887
- Jiang Y et al. LINC01426 contributes to clear cell renal cell carcinoma progression by modulating CTBP1/miR-423-5p/FOXM1 axis via interacting with IGF2BP1. J Cell Physiol 236:427-439 (2021). PubMed: 32583425
- Liang X et al. Comparative Proteomic Analysis of tPVAT during Ang II Infusion. Biomedicines 9:N/A (2021). PubMed: 34944635
- Deng Y et al. Protocatechuic Aldehyde Represses Proliferation and Migration of Breast Cancer Cells through Targeting C-terminal Binding Protein 1. J Breast Cancer 23:20-35 (2020). PubMed: 32140267
- Deng Y et al. CtBP1 transactivates RAD51 and confers cisplatin resistance to breast cancer cells. Mol Carcinog 59:512-519 (2020). PubMed: 32124501