製品の概要

  • 製品名Anti-CSRP3 antibody
    CSRP3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CSRP3
  • 特異性This antibody is specific for CSRP3.
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Pig
  • 免疫原

    A region within synthetic peptide: QGAGCLSTDT GEHLGLQFQQ SPKPARSVTT SNPSKFTAKF GESEKCPRCG, corresponding to amino acids 75-124 of Human CSRP3

  • ポジティブ・コントロール
    • Jurkat cell lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab42504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.125 µg/ml. Predicted molecular weight: 21 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Positive regulator of myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation.
  • 組織特異性Cardiac and slow-twitch skeletal muscles.
  • 関連疾患Defects in CSRP3 are the cause of cardiomyopathy dilated type 1M (CMD1M) [MIM:607482]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in CSRP3 are the cause of cardiomyopathy familial hypertrophic type 12 (CMH12) [MIM:612124]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • 配列類似性Contains 2 LIM zinc-binding domains.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Mainly cytoplasmic (By similarity). In the nucleus it associates with the actin cytoskeleton (Potential). In the Z line, found associated with GLRX3.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cardiac antibody
    • Cardiac LIM protein antibody
    • CLP antibody
    • CMD1M antibody
    • CMH12 antibody
    • CRP3 antibody
    • Csrp3 antibody
    • CSRP3_HUMAN antibody
    • Cysteine and glycine-rich protein 3 antibody
    • Cysteine rich protein 3 antibody
    • Cysteine-rich protein 3 antibody
    • LIM domain only 4 antibody
    • LIM domain protein antibody
    • LMO4 antibody
    • MLP antibody
    • Muscle LIM protein antibody
    see all

Anti-CSRP3 antibody 画像

  • IHC image of CSRP3 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42504, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab42504 staining CSRP3 in Rat neonatal cardiac myocyte cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA for 60 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal(1/500) was used as the secondary antibody.

    See Abreview

  • Anti-CSRP3 antibody (ab42504) at 0.125 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 21 kDa

Anti-CSRP3 antibody (ab42504) 使用論文

This product has been referenced in:
  • Roberts MD  et al. Early depression of Ankrd2 and Csrp3 mRNAs in the polyribosomal and whole tissue fractions in skeletal muscle with decreased voluntary running. J Appl Physiol 112:1291-9 (2012). WB ; Rat . Read more (PubMed: 22282489) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Neonatal cardiac myocytes)
Specification Neonatal cardiac myocytes
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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投稿 Apr 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Cardiac myocytes)
Loading amount 10 µg
Specification Cardiac myocytes
Gel Running Conditions Reduced Denaturing (10 % gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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投稿 Apr 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"