製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CSRP2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Horse, Cow, Opossum
  • 免疫原

    Synthetic peptide:

    C-ERLGIKPESVQP

    , corresponding to internal sequence amino acids 86-97 of Human CSRP2

  • ポジティブ・コントロール
    • Human heart and kidney lysates. Mouse and rat kidney lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab65946 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 21 kDa).

Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529).

IHC-P Use a concentration of 3 - 6 µg/ml.

ターゲット情報

Anti-CSRP2 antibody 画像

  • All lanes : Anti-CSRP2 antibody (ab65946) at 0.2 µg/ml

    Lane 1 : Human kidney lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat kidney lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 21 kDa

Anti-CSRP2 antibody (ab65946) 使用論文

ab65946 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Vascular Smooth Muscle)
Loading amount
20 µg
Specification
Vascular Smooth Muscle
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris with MOPS running buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Jul 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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