製品の概要

  • 製品名Anti-CSEN antibody
    CSEN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CSEN
  • 特異性ab61770 detects endogenous levels of total CSEN protein
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthesized non-phosphopeptide derived from human CSEN around the phosphorylation site of serine 63 (D-S-SP-D-S).

  • ポジティブ・コントロール
    • Human brain tissue; extracts from A431 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception.
    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, such as KCND2/Kv4.2 and KCND3/Kv4.3. Modulates channel expression at the cell membrane, gating characteristics, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner.
    May play a role in the regulation of PSEN2 proteolytic processing and apoptosis. Together with PSEN2 involved in modulation of beta-amyloid formation.
  • 組織特異性Highly expressed in brain. Widely expressed at lower levels. Expression levels are elevated in brain cortex regions affected by Alzheimer disease.
  • 配列類似性Belongs to the recoverin family.
    Contains 4 EF-hand domains.
  • 翻訳後修飾Palmitoylated. Palmitoylation enhances association with the plasma membrane.
    Proteolytically cleaved by caspase-3.
    Phosphorylation at Ser-63 inhibits cleavage by CASP3.
  • 細胞内局在Cytoplasm. Cell membrane. Endoplasmic reticulum. Golgi apparatus. Nucleus. Also membrane-bound, associated with the plasma membrane (PubMed:15485870). In the presence of PSEN2 associated with the endoplasmic reticulum and Golgi. The sumoylated form is present only in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 4933407H12Rik antibody
    • A type potassium channel modulatory protein 3 antibody
    • A-type potassium channel modulatory protein 3 antibody
    • AI413860 antibody
    • Calsenilin antibody
    • CSEN antibody
    • CSEN_HUMAN antibody
    • DRE antagonist modulator antibody
    • DRE-antagonist modulator antibody
    • DREAM antibody
    • EF hand transcription factor antibody
    • KCHIP 3 antibody
    • KChIP3 antibody
    • KCNIP 3 antibody
    • Kcnip3 antibody
    • Kv channel interacting protein 3 antibody
    • Kv channel-interacting protein 3 antibody
    • MGC18289 antibody
    • Potassium channel interacting protein 3 antibody
    • R74849 antibody
    see all

Anti-CSEN antibody 画像

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61770 at a dilution of 1/50. left: untreated sample. Right: sample treated with immunising peptide.
  • All lanes : Anti-CSEN antibody (ab61770) at 1/500 dilution

    Lane 1 : extracts from A431 cells with no peptide
    Lane 2 : extracts from A431 cells with peptide


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
  • ICC/IF image of ab61770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61770, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CSEN antibody (ab61770) 使用論文

ab61770 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Escherichia coli Purified protein (E.Coli)
Loading amount 0.05 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"