製品の概要

  • 製品名Anti-CSAD antibody
    CSAD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CSAD
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse CSAD.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: Liver (Mouse) Tissue; Kidney (Mouse) Tissue; Liver (Rat) Tissue; Kidney (Rat) Tissue; NIH 3T3 Whole Cell. IHC-P (FFPE): Rat Kidney (Normal)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab91016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

Anti-CSAD antibody 画像

  • IHC image of CSAD staining in Rat Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab91016, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-CSAD antibody (ab91016) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Human liver tissue lysate
    Lane 3 : Rat liver tissue lysate
    Lane 4 : Mouse kidney tissue lysate

    Lysates/proteins at 50 µg per lane.


    Performed under reducing conditions.

    Predicted band size : 55 kDa


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Hua Jiang

    See Abreview

  • All lanes : Anti-CSAD antibody (ab91016) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate
    Lane 4 : Kidney (Rat) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 100 kDa,45 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab91016 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab91016 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (5min) PC12 cells at 5ug/ml.

Anti-CSAD antibody (ab91016) 使用論文

ab91016 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Excellent
Abreviews
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12 % gel)
Sample Mouse Tissue lysate - other (liver and kidney)
Specification liver and kidney
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Hua Jiang

Verified customer

投稿 Jun 17 2013

Thank you for your enquiry.

The lab used 5% BSA for testing purposes and for the picture display on this webpage.



Please let us know if you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"