Anti-CRY2 抗体 (ab93802)
Key features and details
- Rabbit polyclonal to CRY2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CRY2 antibody
CRY2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CRY2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide, corresponding to a region within amino acids 543-593 of Human CRY2 (AAH41814.1)
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ポジティブ・コントロール
- Whole cell lysate from HeLa and 293T cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93802の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 67 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 67 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL. -
組織特異性
Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. -
配列類似性
Belongs to the DNA photolyase class-1 family.
Contains 1 DNA photolyase domain. -
翻訳後修飾
Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation. -
細胞内局在
Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL. - Information by UniProt
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参照データベース
- Entrez Gene: 1408 Human
- Omim: 603732 Human
- SwissProt: Q49AN0 Human
- Unigene: 532491 Human
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別名
- cry2 antibody
- CRY2_HUMAN antibody
- cryptochrome 2 (photolyase like) antibody
see all
画像
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All lanes : Anti-CRY2 antibody (ab93802) at 0.04 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Lane 4 : whole 293T cell lysate at 50 µg
Predicted band size: 67 kDa -
Immunoprecipitation analysis of CRY2 expression in whole HeLa cells lysate (1 mg for IP, 20% of IP loaded) using ab93802 at 3 ug/mg lysate (Left Lane). Right lane represent IP antibody control. Subsequent WB analysis was performed using 1ug/ml ab93802.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab93802 は 1 報の論文で使用されています。
- Gorelik M et al. A Structure-Based Strategy for Engineering Selective Ubiquitin Variant Inhibitors of Skp1-Cul1-F-Box Ubiquitin Ligases. Structure 26:1226-1236.e3 (2018). PubMed: 30033217