製品の概要

  • 製品名Anti-CRY2 antibody
    CRY2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CRY2
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Generated from rabbits immunized with a KLH conjugated synthetic peptide between 564~593 amino acids from the C-terminal region of human CRY2.

  • ポジティブ・コントロール
    • Mouse liver tissue lysate, Human breast carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能Blue light-dependent regulator of the circadian feedback loop. Inhibits CLOCK
    NPAS2-ARNTL E box-mediated transcription. Acts, in conjunction with CRY2, in maintaining period length and circadian rhythmicity. Has no photolyase activity. Capable of translocating circadian clock core proteins such as PER proteins to the nucleus. May inhibit CLOCK
    NPAS2-ARNTL transcriptional activity through stabilizing the unphosphorylated form of ARNTL.
  • 組織特異性Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle.
  • 配列類似性Belongs to the DNA photolyase class-1 family.
    Contains 1 DNA photolyase domain.
  • 翻訳後修飾Phosphorylation on Ser-266 by MAPK is important for the inhibition of CLOCK-ARNTL-mediated transcriptional activity. Phosphorylation by CSKNE requires interaction with PER1 or PER2.
    Ubiquitinated by the SCF(FBXL3) and SCF(FBXL21) complex, leading to its degradation.
  • 細胞内局在Cytoplasm. Nucleus. Translocated to the nucleus through interaction with other Clock proteins such as PER2 or ARNTL.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cry2 antibody
    • CRY2_HUMAN antibody
    • cryptochrome 2 (photolyase like) antibody
    • Cryptochrome 2 antibody
    • Cryptochrome-2 antibody
    • FLJ10332 antibody
    • growth inhibiting protein 37 antibody
    • HCRY2 antibody
    • KIAA0658 antibody
    • PHLL2 antibody
    • Photolyase like antibody
    see all

Anti-CRY2 antibody 画像

  • Anti-CRY2 antibody (ab38872) at 1/100 dilution + Mouse liver tissue lysate

    Predicted band size : 67 kDa
    Observed band size : 67 kDa
    Additional bands at : 30 kDa,48 kDa. We are unsure as to the identity of these extra bands.
  • ab38872, at 1/50 dilution, staining CRY2 in Human breast carcinoma by Immunohistochemistry, Formalin fixed and Paraffin embedded tissue. ab38872 was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • ICC/IF image of ab38872 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38872, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-CRY2 antibody (ab38872) 使用論文

This product has been referenced in:
  • Griesauer I  et al. Circadian abnormalities in a mouse model of high trait anxiety and depression. Ann Med 46:148-54 (2014). WB ; Mouse . Read more (PubMed: 24405329) »
  • Li DQ  et al. Metastasis-associated protein 1 is an integral component of the circadian molecular machinery. Nat Commun 4:2545 (2013). Mouse . Read more (PubMed: 24089055) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Liver)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 5 µg
Specification Liver
Blocking step 5%BSA/1%OvaAlb/PBS as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Apr 04 2016


I am very pleased to hear you would like to accept our offer and test ab104736 and ab38872 in IHC-Fr. This code will give you: 1 free primary antibody OR $337.00 OFF OF ORDER for each antibody tested before the expiration date. To redeem this offe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 25 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"