製品の概要

  • 製品名Anti-CRSP2 / MED14 antibody
    CRSP2 / MED14 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CRSP2 / MED14
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 1 and 50 of human CRSP2/ MED14.

  • ポジティブ・コントロール
    • Whole cell lysate from Hela cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at 2-5µg/mg of lysate.
    WB: 1/2000 - 1/10000. Detects bands of approximately 71 and 160 kDa and various other bands (predicted molecular weight: 160 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the Mediator complex subunit 14 family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activator-recruited cofactor 150 kDa component antibody
      • ARC150 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 2 antibody
      • CRSP complex subunit 2 antibody
      • DRIP150 antibody
      • EXLM1 antibody
      • hRGR1 antibody
      • MED14 antibody
      • MED14_HUMAN antibody
      • Mediator complex subunit 14 antibody
      • Mediator of RNA polymerase II transcription subunit 14 antibody
      • RGR1 antibody
      • RGR1 homolog antibody
      • Thyroid hormone receptor-associated protein complex 170 kDa component antibody
      • Transcriptional coactivator CRSP150 antibody
      • Trap170 antibody
      • Vitamin D3 receptor-interacting protein complex 150 kDa component antibody
      see all

    Anti-CRSP2 / MED14 antibody 画像

    • All lanes : Anti-CRSP2 / MED14 antibody (ab72141) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from Hela cells at 50 µg
      Lane 2 : Whole cell lysate from Hela cells at 15 µg
      Lane 3 : Whole cell lysate from Hela cells at 5 µg


      Predicted band size : 160 kDa
      Observed band size : 160 kDa
      Additional bands at : 120 kDa,71 kDa. We are unsure as to the identity of these extra bands.
    • Immunoprecipitation/ Western Blot of CRSP2 / MED14.
      Lane 1: ab72141 at 3µg/mg whole cell lysate for IP.
      Lane 2: Control IgG.

      ab72141 at 1µg/ml for subsequent WB.
      Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
      Detection:Chemiluminescence with an exposure time of 3 seconds.

    Anti-CRSP2 / MED14 antibody (ab72141) 使用論文

    This product has been referenced in:
    • Ruiz A  et al. Characterization of the influence of mediator complex in HIV-1 transcription. J Biol Chem 289:27665-76 (2014). Human . Read more (PubMed: 25100719) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab72141.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"