The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2 - 5 µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing.
Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung.
Belongs to the Luc7 family.
Phosphorylated in vitro by SRPK1, SRPK2 and CLK1.
Nucleus speckle. The subnuclear localization is affected by cisplatin.
Detection of human CROP in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab80415 at 3 µg/mg lysate for IP (lane 1). Lane 2 represents control IgG IP. Subsequent WB detection used ab80412 (which recognizes an upstream epitope) at 1 µg/ml.