製品の概要

  • 製品名Anti-CROCC antibody
    CROCC 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CROCC
  • アプリケーション適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: VKRLEKQNLE KDQVNKDLTE KLEALESLRL QEQAALETED GEGLQQTLRD LAQAVLSDSE SGVQLSGSER TADASNGS, corresponding to amino acids 406-483 of Human CROCC

  • ポジティブ・コントロール
    • Human fallopian tube tissue; U2OS cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

ターゲット情報

  • 機能Major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells which originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. Contributes to centrosome cohesion before mitosis.
  • 配列類似性Belongs to the rootletin family.
  • 翻訳後修飾Phosphorylated by NEK2 which may regulate its association with centrosomes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. In ciliated cells, associated with ciliary rootlets. In non-ciliated cells, localized between, around and at the proximal ends of the centrioles. Dissociates from the centrioles at the onset of mitosis and reassociates with them at anaphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ciliary rootlet coiled coil antibody
    • Ciliary rootlet coiled-coil protein antibody
    • Crocc antibody
    • CROCC_HUMAN antibody
    • KIAA0445 antibody
    • Rootletin antibody
    see all

Anti-CROCC antibody 画像

  • ab121653, at 1/150 dilution, staining CROCC in Human fallopian tube tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • ab121653 at 4 µg/ml staining CROCC in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence (green).

Anti-CROCC antibody (ab121653) 使用論文

ab121653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"