Mouse monoclonal [2212C1a] to CRNKL1
Recombinant human CRNKL1 fragment
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4).
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Protein G purified
Ab51348 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 100 kDa.
Use at an assay dependent dilution.
Involved in pre-mRNA splicing process.
Widely expressed. Highly expressed in testis. Not expressed in brain and lung.
Belongs to the crooked-neck family.
Contains 17 HAT repeats.
Nucleus speckle. Cytoplasm. Colocalizes with core spliceosomal snRNP proteins. Also diffusely expressed in cytoplasm.
Information by UniProt
Western blot - Anti-CRNKL1 antibody [2212C1a] (ab51348)
Immunocytochemistry/ Immunofluorescence - Anti-CRNKL1 antibody [2212C1a] (ab51348) This image is a courtesy of an anonymous Abreview.
ab51348 staining CRNKL1 in Human glioblastoma cells by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 01.% Triton X100 in PBS prior to blocking in 0.5% BSA for 20 minutes at room temperature. The primary antibody was diluted 1/50 in 0.5% BSA in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor
® 488-conjugated Goat anti-Mouse polyclonal, diluted 1/400. Nuclei were counterstained with Hoechst (blue)
has not yet been referenced specifically in any publications.
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