製品の概要

  • 製品名Anti-CRMP2 (phospho T514) antibody
    CRMP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CRMP2 (phospho T514)
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human CRMP2, phosphorylated at T514.

    (Peptide available as ab99478.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in human, mouse and rat brain tissue lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85934 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 翻訳後修飾3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Anti-CRMP2 (phospho T514) antibody 画像

  • All lanes : Anti-CRMP2 (phospho T514) antibody (ab85934) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 5 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 6 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Phosphorylated T 514)
    Lane 7 : Human brain tissue lysate - total protein (ab29466) with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 8 : Brain (Mouse) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)
    Lane 9 : Brain (Rat) Tissue Lysate with Human Dihydropyrimidinase-related protein 2 Synth (508 - 520) (Non-Modified)

    Lysates/proteins at 10 µg per lane.
    Blocking peptides at 1 µg/ml per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 62,70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • ICC/IF image of ab85934 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85934 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • CRMP2 was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to CRMP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab85934.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 70kDa; CRMP2

Anti-CRMP2 (phospho T514) antibody (ab85934) 使用論文

This product has been referenced in:
  • Akama K  et al. Proteomic identification of differentially expressed genes in neural stem cells and neurons differentiated from embryonic stem cells of cynomolgus monkey (Macaca fascicularis) in vitro. Biochim Biophys Acta 1814:265-76 (2011). WB ; Monkey . Read more (PubMed: 21047566) »

See 1 Publication for this product

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