製品の概要

  • 製品名Anti-CRMP2 antibody [1B1]
    CRMP2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1B1] to CRMP2
  • アプリケーション適用あり: Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Immunogen: A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator protein Type 2 (MW: 65 kDa).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/5000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
IHC-P 1/100 - 1/1000.

ターゲット情報

  • 機能Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
  • 翻訳後修飾3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Collapsin response mediator protein 2 antibody
    • Collapsin response mediator protein antibody
    • Collapsin response mediator protein hCRMP 2 antibody
    • CRAM antibody
    • CRMP 2 antibody
    • CRMP-2 antibody
    • CRMP2 antibody
    • DHPRP 2 antibody
    • DHPRP2 antibody
    • Dihydropyrimidinase 2 antibody
    • Dihydropyrimidinase like 2 antibody
    • Dihydropyrimidinase like 2 long form antibody
    • Dihydropyrimidinase related protein 2 antibody
    • Dihydropyrimidinase-related protein 2 antibody
    • DPYL 2 antibody
    • DPYL2 antibody
    • DPYL2_HUMAN antibody
    • DPYSL 2 antibody
    • Dpysl2 antibody
    • DRP-2 antibody
    • DRP2 antibody
    • Musunc 33 antibody
    • Musunc33 antibody
    • N2A3 antibody
    • TOAD 64 antibody
    • TOAD64 antibody
    • ULIP 2 protein antibody
    • ULIP-2 antibody
    • Ulip2 antibody
    • Unc-33-like phosphoprotein 2 antibody
    see all

Anti-CRMP2 antibody [1B1] 画像

  • Anti-CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + CRMP2 antibody [1B1] (ab62539) at 1/2500 dilution + SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 65 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing SH-SY5Y cells stained with ab62539 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62539, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-CRMP2 antibody [1B1] (ab62539) 使用論文

This product has been referenced in:
  • Zhou K  et al. NMDA receptor hypofunction induces dysfunctions of energy metabolism and semaphorin signaling in rats: a synaptic proteome study. Schizophr Bull 38:579-91 (2012). WB ; Rat . Read more (PubMed: 21084551) »
  • Chi XX  et al. Regulation of N-type voltage-gated calcium channels (Cav2.2) and transmitter release by collapsin response mediator protein-2 (CRMP-2) in sensory neurons. J Cell Sci 122:4351-62 (2009). Read more (PubMed: 19903690) »

See all 2 Publications for this product

Product Wall

The formulation is 1% sucrose with Tris 0.05M and glycine 0.05M. The antibody formulation does not contain preservative or glycerol.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"