製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to CrkRS
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: LVEGDLSSAP QELNPAVTAA LLQLLSQPEA EPPGHLPHEH QALRPMEYST RPRPNRTYGN TDGPETGFSA IDTDERNSGP ALTESLVQTL VKNRTFSGSL , corresponding to amino acids 1281-1380 of Human CrkRS

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57311 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  • 組織特異性
    Ubiquitously expressed.
  • 関連疾患
    Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
  • 配列類似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylation at Thr-893 increases kinase activity.
  • 細胞内局在
    Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • arginine/serine-rich antibody
    • Cdc2 related kinase arginine/serine rich antibody
    • CDC2 related protein kinase 7 antibody
    • Cdc2-related kinase antibody
    • CDC2-related protein kinase 7 antibody
    • Cdk12 antibody
    • CDK12_HUMAN antibody
    • Cell division cycle 2 related protein kinase 7 antibody
    • Cell division cycle 2-related protein kinase 7 antibody
    • Cell division protein kinase 12 antibody
    • CRK7 antibody
    • CRKR antibody
    • CrkRS antibody
    • Cyclin-dependent kinase 12 antibody
    • EC 2.7.11.22 antibody
    • hCDK12 antibody
    • kiaa0904 antibody
    • Pksc antibody
    see all

画像

  • CrkRS antibody (ab57311) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57311 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57311, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:
  • Eifler TT  et al. Cyclin-dependent kinase 12 increases 3' end processing of growth factor-induced c-FOS transcripts. Mol Cell Biol 35:468-78 (2015). WB, IP . Read more (PubMed: 25384976) »
  • Ekumi KM  et al. Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex. Nucleic Acids Res 43:2575-89 (2015). ChIP . Read more (PubMed: 25712099) »

See all 3 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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投稿 Nov 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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