製品の概要

  • 製品名Anti-CrkRS antibody
    CrkRS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to CrkRS
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the sequence of Human CrkRS within amino acids 1-100.

  • ポジティブ・コントロール
    • Mouse liver tissue lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody was purified through a protein G column and eluted out with both high and low pH buffers. It was neutralized immediately after elution, followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 164 kDa).

ターゲット情報

  • 機能Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Chromosomal aberrations involving CDK12 may be a cause gastric cancer. Deletions within 17q12 region producing fusion transcripts with ERBB2, leading to CDK12-ERBB2 fusion leading to trunctated CDK12 protein not in-frame with ERBB2.
  • 配列類似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation at Thr-893 increases kinase activity.
  • 細胞内局在Nucleus. Nucleus speckle. Colocalized with nuclear speckles throughout interphase.
  • Information by UniProt
  • 参照データベース
  • 別名
    • arginine/serine-rich antibody
    • Cdc2 related kinase arginine/serine rich antibody
    • CDC2 related protein kinase 7 antibody
    • Cdc2-related kinase antibody
    • CDC2-related protein kinase 7 antibody
    • Cdk12 antibody
    • CDK12_HUMAN antibody
    • Cell division cycle 2 related protein kinase 7 antibody
    • Cell division cycle 2-related protein kinase 7 antibody
    • Cell division protein kinase 12 antibody
    • CRK7 antibody
    • CRKR antibody
    • CrkRS antibody
    • Cyclin-dependent kinase 12 antibody
    • EC 2.7.11.22 antibody
    • hCDK12 antibody
    • kiaa0904 antibody
    • Pksc antibody
    see all

Anti-CrkRS antibody 画像

Anti-CrkRS antibody (ab37914) 使用論文

ab37914 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS
Username

Dr. Kirk McManus

Verified customer

投稿 Jun 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"