Anti-Crk p38 (phospho Y221) 抗体 (ab51222)

製品の概要

  • 製品名
    Anti-Crk p38 (phospho Y221) antibody
    Crk p38 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Crk p38 (phospho Y221)
  • 特異性
    This antibody detects endogenous levels of CRK isoform II only when phosphorylated at tyrosine 221.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    A synthesized phosphopeptide derived from human CRK isoform II around the phosphorylation site of tyrosine 221 (G-P-YP-A-Q).

  • ポジティブ・コントロール
    • Extracts of COS7 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab51222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).
ELISA 1/40000.

ターゲット情報

  • 機能
    The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • 配列類似性
    Belongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • ドメイン
    The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
    The SH2 domain mediates interaction with SHB.
  • 翻訳後修飾
    Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
    Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
  • 細胞内局在
    Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adapter molecule crk antibody
    • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
    • CRK antibody
    • CRK isoform 2 antibody
    • CRK isoform II antibody
    • CRK_HUMAN antibody
    • CRKII antibody
    • FLJ38130 antibody
    • OTTHUMP00000115366 antibody
    • OTTHUMP00000198330 antibody
    • p38 antibody
    • Proto oncogene C crk antibody
    • Proto-oncogene C-crk antibody
    • v crk avian sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    see all

画像

  • All lanes : Anti-Crk p38 (phospho Y221) antibody (ab51222) at 1/300 dilution

    Lane 1 : Extracts of cos7 cells treated with phosphopeptide
    Lane 2 : Extracts of cos7 cells


    Predicted band size : 34 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab51222 has not yet been referenced specifically in any publications.

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