製品の概要

  • 製品名Anti-Crk p38 antibody
    Crk p38 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Crk p38
  • 特異性ab51211 detects endogenous levels of total CrkII protein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized non-phosphopeptide derived from human CrkII around the phosphorylation site of tyrosine 221(G-P-YP-A-Q).

  • ポジティブ・コントロール
    • Extracts from cos7 cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/300 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 34 kDa).
ELISA 1/10000.

ターゲット情報

  • 機能The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.
  • 配列類似性Belongs to the CRK family.
    Contains 1 SH2 domain.
    Contains 2 SH3 domains.
  • ドメインThe C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
    The SH2 domain mediates interaction with SHB.
  • 翻訳後修飾Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
    Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.
  • 細胞内局在Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adapter molecule crk antibody
    • Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
    • CRK antibody
    • CRK isoform 2 antibody
    • CRK isoform II antibody
    • CRK_HUMAN antibody
    • CRKII antibody
    • FLJ38130 antibody
    • OTTHUMP00000115366 antibody
    • OTTHUMP00000198330 antibody
    • p38 antibody
    • Proto oncogene C crk antibody
    • Proto-oncogene C-crk antibody
    • v crk avian sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog antibody
    • v crk sarcoma virus CT10 oncogene homolog (avian) antibody
    see all

Anti-Crk p38 antibody 画像

  • All lanes : Anti-Crk p38 antibody (ab51211)

    Lane 1 : Extracts from cos7 cells with no immunising peptide
    Lane 2 : Extracts from cos7 cells with immunising peptide


    Predicted band size : 34 kDa

Anti-Crk p38 antibody (ab51211) 使用論文

ab51211 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab51211.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"