Anti-Crk p38 抗体 [2210C1a] (ab51347)
Key features and details
- Mouse monoclonal [2210C1a] to Crk p38
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
製品の概要
-
製品名
Anti-Crk p38 antibody [2210C1a]
Crk p38 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2210C1a] to Crk p38 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant human CRK
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading...
-
精製度
Protein G purified -
特記事項(精製)
Ab51347 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
2210C1a -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51347の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Predicted molecular weight: 34 kDa. |
ターゲット情報
-
機能
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. -
配列類似性
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. -
細胞内局在
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion. - Information by UniProt
-
別名
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
see all
画像
-
Anti-Crk p38 antibody [2210C1a] (ab51347) + Recombinant Crk p38
Predicted band size: 34 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab51347 は論文での使用が確認できていません。