Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling CRIP2 with ab83489 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - CRIP2. Right - Merge.
Western blot - CRIP2 antibody (ab83489)
Anti-CRIP2 antibody (ab83489) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 50000
Predicted band size : 22 kDa Observed band size : 22 kDa Gel concentration: 12%
Western blot - Anti-CRIP2 antibody (ab83489)
Predicted band size : 22 kDa Western blot analysis of MCF7 cell lysate labeling CRIP2 with ab83489 at 1.0µg/ml.