Anti-CRF1/CRHR1 抗体 (ab77686)
Key features and details
- Goat polyclonal to CRF1/CRHR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CRF1/CRHR1 antibody
CRF1/CRHR1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CRF1/CRHR1 -
由来種
Goat -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rhesus monkey -
免疫原
Synthetic peptide corresponding to Human CRF1/CRHR1 aa 107-117 (internal sequence).
Sequence:C-NEEKKSKVHYH
Database link: NP_004373.2 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab77686 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77686の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use a concentration of 0.3 µg/ml.
|
特記事項 |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 0.3 µg/ml. |
ターゲット情報
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機能
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. -
組織特異性
Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. -
翻訳後修飾
C-terminal Ser or Thr residues may be phosphorylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1394 Human
- Entrez Gene: 12921 Mouse
- Entrez Gene: 58959 Rat
- Omim: 122561 Human
- SwissProt: P34998 Human
- SwissProt: P35347 Mouse
- SwissProt: P35353 Rat
- Unigene: 417628 Human
see all -
別名
- corticotropin releasing factor receptor antibody
- Corticotropin releasing factor type 1 receptor antibody
- corticotropin releasing hormone receptor 1 antibody
see all
画像
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All lanes : Anti-CRF1/CRHR1 antibody (ab77686) at 1 µg/ml
Lane 1 : Human colon tissue lysate in RIPA buffer
Lane 2 : Human cerebellum tissue lysate in RIPA buffer
Lane 3 : Rat brain tissue lysate in RIPA buffer
Lane 4 : Mouse spinal cord tissue lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDaIncubated with the primary antibody for 1 hour. Detected by chemiluminescence.
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Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody was used as the secondary antibody (green) showing cytoplasmic, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10μg/mL) followed by Alexa Fluor® 488 secondary antibody (2μg/mL).
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Immunocytochemistry/immunofluorescence analysis of Neuro2a cells labelling CRF1 with ab77686 at 10µg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.15% Triton X-100. Anti-goat Alexa Fluor® 488 antibody at 2ug/ml was used as the secondary antibody (green) showing plasma membrane, and vesicle staining. Nuclear DNA was labelled with DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor® 488 secondary antibody (2ug/ml).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab77686 は 5 報の論文で使用されています。
- Yamanaka-Takaichi M et al. Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa. Int J Mol Sci 22:N/A (2021). PubMed: 33803422
- You X et al. Urocortins exhibit differential effects on PGE2 and PGF2α output via CRHR2 in human myometrium. Reproduction 162:11-20 (2021). PubMed: 33929340
- Ayyadurai S et al. Frontline Science: Corticotropin-releasing factor receptor subtype 1 is a critical modulator of mast cell degranulation and stress-induced pathophysiology. J Leukoc Biol 102:1299-1312 (2017). PubMed: 28684600
- Wierzbicka JM et al. Differentiation of Keratinocytes Modulates Skin HPA Analog. J Cell Physiol 232:154-66 (2017). PubMed: 27061711
- Wierzbicka JM et al. Bioactive forms of vitamin D selectively stimulate the skin analog of the hypothalamus-pituitary-adrenal axis in human epidermal keratinocytes. Mol Cell Endocrinol 437:312-322 (2016). PubMed: 27524410