製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to CRF1
  • アプリケーション
    適用あり: IHC-P, ELISA, IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Sheep, Chicken, Human, Pufferfish, Rhesus monkey, African green monkey, Syrian hamster
  • 免疫原

    Synthetic peptide corresponding to Human CRF1 aa 279-292 (internal sequence) (Cysteine residue). Synthetic peptide:

    KLYYDNEKCWFGKR

    by a Cysteine residue linker, corresponding to internal sequence amino acids 279-292 of Human CRF1(Peptide available as ab113546.)
    Sequence:

    KLYYDNEKCWFGKR


    (Peptide available as ab113546)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.
ELISA 1/16000. (Peptide)
IHC-FoFr Use at an assay dependent dilution. PubMed: 20383137
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human CRF1 peptide (ab113546).

ターゲット情報

  • 機能
    This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • 組織特異性
    Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family.
  • 翻訳後修飾
    C-terminal Ser or Thr residues may be phosphorylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Corticotropin releasing factor type 1 receptor antibody
    • corticotropin releasing hormone receptor 1 antibody
    • Corticotropin-releasing factor receptor 1 antibody
    • Corticotropin-releasing hormone receptor 1 antibody
    • CRF R antibody
    • crf receptor 1 antibody
    • crf receptor type 1 antibody
    • crf type 1 antibody
    • CRF-R antibody
    • CRF-R-1 antibody
    • CRF-R1 antibody
    • CRF1 antibody
    • CRFR antibody
    • CRFR-1 antibody
    • CRFR1 antibody
    • CRFR1_HUMAN antibody
    • CRH-R-1 antibody
    • CRH-R1 antibody
    • CRH-R1h antibody
    • CRHR antibody
    • Crhr1 antibody
    • CRHR1f antibody
    • CRHR1L antibody
    see all

Anti-CRF1 antibody 画像

  • ab59023 (1/100) staining CRF1 in paraffin embedded mouse brain tissue sections. Tissues were fixed in paraformaldehyde and permeabilised with 0.3% Triton X100/TBS. Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-CRF1 antibody (ab59023) at 1 µg/ml

    Lane 1 : Rat Forebrain
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 50 kDa
    Additional bands at : 110 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-CRF1 antibody (ab59023) 使用論文

This product has been referenced in:
  • Zheng LL  et al. Preimplantation maternal stress impairs embryo development by inducing oviductal apoptosis with activation of the Fas system. Mol Hum Reprod 22:778-790 (2016). Read more (PubMed: 27475493) »
  • Johnson AC  et al. Knockdown of corticotropin-releasing factor in the central amygdala reverses persistent viscerosomatic hyperalgesia. Transl Psychiatry 5:e517 (2015). Read more (PubMed: 25734510) »

See all 5 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 22 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (Gradient)
Blocking step
Casein as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton-X
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 15 2012

Thank you for contacting us.

As we discussed on the phone, adding a BSA block and trying a shorter antigen retrieval time may help to reduce the background for ab7963, ab59023, and ab75168, while trying a 1:100 dilution for ab35748 may help t...

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DISCOUNT CODES :
**** for ab1376
**** for ab59023
Expiration date: DD MM YYYY for both codes

I am very pleased to hear you would like to accept our offer and test ab1376 and ab59023 in Pig.Each code will give you 1 freeprimary...

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Thank you for your inquiry.
Unfortunately, this antibody was not tested with samples from a knock out mouse in WB by our lab. We also do not have any data/information about other customers using this antibody in CRF1 ko mice.
I am sorry I did...

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Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

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I am sorry that you are having difficulties using some of our antibodies in western blot. From the data on the website, it does appear that in addition to recognizing the bands of interest, for ab59023 a 50kDa band and for ab75168, a band at 48kDa, bo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain slices)
Specification
Brain slices
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Christoph Schwarzer

Verified customer

投稿 Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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