製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to CREM
  • アプリケーション
    適用あり: IHC-Fr, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from an internal sequence of human CREM.

  • ポジティブ・コントロール
    • K562 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 21383078
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 37 kDa).

ターゲット情報

  • 機能
    Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
  • 組織特異性
    Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
  • 配列類似性
    Belongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻訳後修飾
    Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
    Stimulated by phosphorylation.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP response element modulator antibody
    • cAMP responsive element modulator antibody
    • cAMP-responsive element modulator antibody
    • CREM antibody
    • CREM-2 antibody
    • CREM_HUMAN antibody
    • hCREM 2 antibody
    • hCREM-2 antibody
    • hCREM2 antibody
    • ICER antibody
    • Inducible cAMP early repressor antibody
    • Inducible cAMP early repressor ICER antibody
    • MGC111110 antibody
    • MGC17881 antibody
    • MGC41893 antibody
    see all

画像

  • All lanes : Anti-CREM antibody (ab64832) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : K562 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa. We are unsure as to the identity of these extra bands.

参考文献

This product has been referenced in:
  • Johansson EM  et al. Sex-related differences of cAMP-specific PDE4B3 mRNA in oligodendrocytes following systemic inflammation. Glia : (2012). WB . Read more (PubMed: 22848007) »
  • Yabuta Y  et al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21383078) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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