製品の概要

  • 製品名Anti-CREM antibody
    CREM 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to CREM
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse
  • 免疫原

    Fusion protein:

    MTMETVESQQDRSVTHSVAEHSSLHMQTGQISVPTLAQVSVAGSGTGRGS PAVTLVQLPSGQTVQVQGVIQTPHPSVIQSPQIQTVQVATIAETDDSADS

    , corresponding to amino acids 1-100 of Rat CREM.

  • 特記事項Produced from outbred CD1 mice


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーConstituents: 50% Glycerol
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab21763 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 32 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.
  • 組織特異性Expressed in testes (round spermatids) (at protein level). Isoform 14 is the major activator form in testes.
  • 配列類似性Belongs to the bZIP family.
    Contains 1 bZIP (basic-leucine zipper) domain.
    Contains 1 KID (kinase-inducible) domain.
  • 翻訳後修飾Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome.
    Stimulated by phosphorylation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP response element modulator antibody
    • cAMP responsive element modulator antibody
    • cAMP-responsive element modulator antibody
    • CREM antibody
    • CREM-2 antibody
    • CREM_HUMAN antibody
    • hCREM 2 antibody
    • hCREM-2 antibody
    • hCREM2 antibody
    • ICER antibody
    • Inducible cAMP early repressor antibody
    • Inducible cAMP early repressor ICER antibody
    • MGC111110 antibody
    • MGC17881 antibody
    • MGC41893 antibody
    see all

Anti-CREM antibody (ab21763) 使用論文

ab21763 has not yet been referenced specifically in any publications.

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