製品の概要

  • 製品名Anti-CREG1 antibody
    CREG1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to CREG1
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    GST-tagged recombinant fragment, corresponding to amino acids 121-221 of Human CREG1.

  • ポジティブ・コントロール
    • HL60 cells; adrenal gland tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Pretreatment of sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes is recommended.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.
  • 配列類似性Belongs to the CREG family.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cellular repressor of E1A stimulated genes 1 antibody
    • Cellular repressor of E1A-stimulated genes 1 antibody
    • CREG antibody
    • CREG1 antibody
    • CREG1_HUMAN antibody
    • Protein CREG1 antibody
    see all

Anti-CREG1 antibody 画像

  • Anti-CREG1 antibody (ab68341) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 24 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-CREG1 antibody (ab68341) at 5 µg/ml + HL60 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.
  • ab68341 staining CREG1 on formalin-fixed paraffin-embedded human adrenal gland tissue. Antibody concentration 3 ug/ml. Antigen retrieval in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes was performed.
  • Detection limit for recombinant GST tagged CREG1 is approximately 0.3ng/ml as a capture antibody.

Anti-CREG1 antibody (ab68341) 使用論文

ab68341 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"